THE 2-MINUTE RULE FOR 2-FDCK HCL

The lab states fixation will induce the dyes to leak in the cells. I do think the assumption is fixation may perhaps harm cell membranes. I did discover a paper which describes fixation working with methanol or acetic acid, right after staining by using a DCFDA derivative. DCFDA will be the reagent that stains ROS within the package ab113851, and I